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. Author manuscript; available in PMC: 2017 Jul 18.
Published in final edited form as: Nature. 2017 Jan 18;541(7638):488–493. doi: 10.1038/nature21049

Figure 4. A peptide communication code guiding lysogeny in Bacillus phages.

Figure 4

(A) Selected instances of AimR homologs in sequenced genomes. Locus tags are indicated for AimR homologs, along with the percent amino acid sequence identity to the phi3T AimR. Mature arbitrium peptide is indicated below the AimP homolog. (B) Distribution of arbitrium peptides among 112 homologs of AimP. (C) Amino acid profile of arbitrium peptide types. (D) Growth curves of B. subtilis BEST7003 infected by spBeta at MOI=0.1, in LB media supplemented with synthesized GMPRGA peptide. The BEST7003 strain was used for spBeta infection as the B. subtilis 168 strain is naturally immune to spBeta. (E) Growth curves of B. subtilis BEST7003 infected by spBeta at MOI=0.1, in LB media supplemented with synthesized SAIRGA peptide. (F) Growth curves of B. subtilis 168 infected by phi3T at MOI=0.1, in LB media supplemented with synthesized GMPRGA peptide. Data in panels D-F represent average of 3 biological replicates, each with 3 technical replicates; error bars represent SE.