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Simultaneous multi-parametric PAM of (A) CHb, (B) sO2, and (C) CBF (both flow speed and direction) in the mouse brain through the intact skull. (D)–(F) are the blow-up views of the boxed regions in (A)–(C), showing the microvasculature resolved by PAM. Scale bars: 1 mm in (A) and 200 μm in (D). The red and blue arrows in panel (C) indicate the directions of the blood flow.
