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Comparison of clearing techniques.
| Method | Clearing composition | Refractive index | Protein fluorescence quencing | Clearing capability | Immunostaining |
|---|---|---|---|---|---|
| Et + BABB | Ethanol Benzyl alcohol Benzyl Benzoate | 1.54 | Yes | Good | No |
| THF + DBE | Thetrahydrofuran Dibenzyl ether | 1.56 | Yes | Good | No |
| SeeDB | Fructose saturated α thioglycerol | 1.49 | No | Moderate | No |
| CUBIC | Urea Aminoalcohols | 1.47 | No | Good | Yes |
| CLARITY | Focus Cl ear™ | 1.45 | No | Good | Yes |
| TDE | 2,2′ Thiodioethanol | Tunable | No | Good | Yes |
