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Cos7 cells transiently-transfected with ANKRD54-wt or ANKRD54-Δ3 mutants followed by immunoprecipitation with streptavidin magnetic-beads. The IP complexes were incubated with whole cell lysates obtained from wt and Btk-KO primary spleen cells. The absence of BTK (second lane from left to right) did not enhance the enrichment to LYN. β-Actin used as quantity-index of protein, indicating equal-loading control of WCL.
