The reproducibility of global and regional strain analysis for cardiac MRI and ultrasound were assessed by both intra- and inter-observer comparisons. Bland-Altman plots showed that systematic biases for global strains (A, C) and regional strains (B, D) from cardiac MRI were smaller than global strains (E, G) and regional strain biases (F, H) from ultrasound. Moreover, the 95% limits of agreement for MRI based strain were narrower than those for ultrasound. These results demonstrate a higher reproducibility of strain analysis from MRI data compared to ultrasound. Ecc: negative peak of circumferential strain.