Figure 4.

Subcellular fractionation confirms impaired CRTC1 nuclear translocation in hippocampus at preplaque AD stages. ( A ) CRTC1-immunoreactive bands were detected in cytosolic and nuclear fractions of hippocampal tissue from wild-type and Alzheimer transgenic rats. ( B, C ) While no significant difference in CRTC1 levels was observed in the cytosolic fractions, a significant reduction in nuclear CRTC1 occurred in the Alzheimer transgenic rats. Cyclophilin A and histone H3 were used as cytosolic and nuclear markers, respectively. Data represent the mean ± SEM. * P < 0.05.