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. Author manuscript; available in PMC: 2017 Apr 3.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2014 May 22;34(7):1531–1538. doi: 10.1161/ATVBAHA.114.303818

Figure 5.

Figure 5

LacZ expression in transgenic mice containing 0.3-kb roundabout 4 (Robo4) proximal promoter. A, Schematic of the wild-type (WT) and deletant (DEL) hypoxanthine phosphoribosyltransferase (Hprt)-targeted alleles. The 3-kb or 0.3-kb human Robo4 promoter, LacZ cDNA, and SV40 polyadenylation signal upstream of the Hprt gene are indicated. B and C, Organs (brain, lung, heart, and diaphragm) were harvested from WT and DEL mice and processed for whole mount and section staining with X-Gal. Arrows indicate the LacZ-expressing endothelial cells in blood vessels of DEL mice.