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. Author manuscript; available in PMC: 2017 Apr 3.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2014 May 22;34(7):1531–1538. doi: 10.1161/ATVBAHA.114.303818

Figure 6.

Figure 6

Differentiation stage–specific DNA methylation of the roundabout 4 (Robo4) promoter in targeted ES cells. A, Targeted ES cells containing the wild-type Robo4 promoter were differentiated, and Flk-1+ and CD31+ cells were separated by magnetic activated cell sorting. The methylation pattern of the targeted Robo4 promoter in undifferentiated and differentiated cells was analyzed by bisulfite sequencing. B and C, Targeted embryonic stem (ES) cells containing the Robo4 promoters with either the −119 ETS mutation (B) or the −42 and −153 SP1 double mutation (C) were differentiated into fetal liver kinase (Flk)-1+ cells. The methylation patterns of the targeted Robo4 promoters were analyzed by bisulfite sequencing.