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. Author manuscript; available in PMC: 2017 Apr 3.
Published in final edited form as: Stem Cells. 2013 Nov;31(11):2408–2419. doi: 10.1002/stem.1502

Figure 4.

Figure 4

Both EOC and EOC-CM injections significantly reduce renal fibrosis in the SNX rat 4 weeks post-therapy initiation. (A, B): Kidney sections were prepared 8 weeks after surgery and immunostained with an antibody specific for type IV collagen. (A): Representative glomerular images with quantitative analysis below. Original magnification ×400. Scale bars = 50 μm. (B): Representative cortical interstitial images with quantitative analysis below. Original magnification ×160. Scale bars = 100 μm. (C, D): RNA isolated from snap-frozen kidney tissue was reverse transcribed and analyzed via quantitative real-time polymerase chain reaction for levels of α (1) type I collagen (C) and α (1) type IV collagen mRNA (D). All values are referenced to levels of Rpl13a, a housekeeper transcript, and are expressed relative to sham-operated animal values. n = 4–9 animals per condition. *, p < .05 versus sham-operated animals; †, p < .05 versus SNX-EBM-2 animals. Abbreviations: AU, arbitrary units; EBM-2, endothelial basal medium-2; EOC-CM, early outgrowth cell conditioned media; SNX, subtotal nephrectomy.