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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: J Cell Physiol. 2017 Feb 24;232(7):1754–1760. doi: 10.1002/jcp.25674

Figure 2. Expression patterns of ERα and ERβ variants differ between asthmatic and non-asthmatic ASM cells.

Figure 2

Total RNA from ASM cells, isolated from non-asthmatic or asthmatic individuals, was reverse transcribed and the cDNA was used in qPCR to measure the expression of various ERα and ERβ isoforms. (A) Expression of all three ERα isoforms (ERα-36, ERα-46 and ERα-FL) were drastically elevated in asthmatic ASM. (B) A similar trend was seen in variants 1–4 of ERβ; however, variant 5 doesn’t seem to be increased in asthmatic ASM. N=4 each group; *indicates significant difference from ‘Non-asthmatic’ ASM; P<0.05.