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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: J Cell Physiol. 2017 Feb 24;232(7):1754–1760. doi: 10.1002/jcp.25674

Figure 4. Inflammation signals regulate the expression of ERα variants in the ASM.

Figure 4

Non-asthmatic ASM cells were treated with either 20ng/ml TNFα or 50ng/ml IL-13 for 24h or 48h before total RNA was isolated and reverse transcribed. The resultant cDNA was subjected to qPCR to measure the expression of various ERα isoforms. (A) While a 24h TNFα treatment increases the expression of ERα-46 and ERα-FL, there was no quantifiable change in ERα-36 expression; IL-13 treatment upregulates the expression of ERα-46 (but not that of the other isoforms) in that time period. (B) The same trend was observed 48h post treatment, as well. N=4, each group; *indicates significant difference from vehicle; P<0.05.