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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: J Cell Physiol. 2017 Feb 24;232(7):1754–1760. doi: 10.1002/jcp.25674

Table 1. Oligonucleotide primers for qPCR on human ASM.

Forward and reverse primers specific for human amplicons targeted in the current study were obtained from Integrated DNA Technologies. Primers were used at a final concentration of 500nM in the quantitative real-time PCR reactions.

Primer ID Sequence
hERα36 5′GAGAATCCTGAACTTGCATCCT3′/ 5′TTAGACACGAGGAAACCACTTG3
hERα46 5′GCTACCATTATGGAGTCTGGTCCTGT 3′/ 5′CCCACCTTTCATCATTCCCACTTCGT 3
hERα-FL 5′TGCAAGGCCTTCTTCAAGAGA3′/ 5′TCAATGGTGCACTGGTTGGT 3
hERβ-V1 5′CTCCCAGCAGCAATCCATGCGCC3′/ 5′AGCAGGTCATACACTGGGACCACA3
hERβ-V2 5′CTCCCAGCAGCAATCCATGCGCC3′/ 5′ TCACTGCTCCATCGTTGCTTCAGG3
hERβ-V3 5′CTCCCAGCAGCAATCCATGCGCC 3′/ 5′TCATTCCCGTGGCTGTTGACAGGC3
hERβ-V4 5′CTCCCAGCAGCAATCCATGCGCC 3′/ 5′CTAAGATAACTTCAAATGAATGAATTG3
hERβ-V5 5′GCTGAACGCCGTGACCGATGCTT 3′/ 5′TTAGGGCGCGTACCTCGCATGCC3
hS16 5′CAATGGTCTCATCAAGGTGAACGG3′/ 5′CTGACGGATAGCATAAATCTGGGC3