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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Exp Physiol. 2017 Mar 14;102(4):397–410. doi: 10.1113/EP086189

Table 2.

Primer sequences for gene expression analysis.

Gene name Primer sequence
Glyceraldehyde 3-phosphate
dehydrogenase (GAPDH) F
5'- GTG TCC GTC GTG GAT CTG 3’
Glyceraldehyde 3-phosphate
dehydrogenase (GAPDH) R
5'- CCT GCT TCA CCA CCT TCT TG 3’
18S F 5' GAT CCA TTG GAG GGC AAG TCT 3'
18S R 5' CCA AGA TCC AAC TAC GAG CTT TTT 3'
Telomere repeat binding factor 1 (TRF1) F 5'-CAT GGA CTA CAC AGA CTT AC-3'
Telomere repeat binding factor 1 (TRF1) R 5'-ATC TGG CCT ATC CTT AGA CG-3'
Telomere repeat binding factor 2 (TRF2) F 5'-TGT CTG TCG CGC ATT GAA GA-3'
Telomere repeat binding factor 2 (TRF2) R 5'-GCT GGA AGA CCT CAA TAG GAA-3'
Protection of telomeres 1a (POT1a) F 5'-CCC TGA ATC TAC TCA AGG AAG-3'
Protection of telomeres 1a (POT1a) R 5'-GAA GCG AAC AAT GTC TCC AA-3'
Protection of telomeres 1b (POT1b) F 5'- CTT TAA GCC TCC GGC CTT AAG CAA
AG-3'
Protection of telomeres 1b (POT1b) R 5'- CTT GGA CAT GAT TAT CAG CAA CGA
CA-3'
mouse telomerase reverse transcriptase
(mTert) F
5'- GCC TTG AGC ACA ATG ACC -3'
mouse telomerase reverse transcriptase
(mTert) R
5'- ATA TCC GTG GTG GCA CAA AT -3'