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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Clin Exp Allergy. 2017 Jan 26;47(4):457–466. doi: 10.1111/cea.12878

Figure 2.

Figure 2

Antibody production in NP and tonsil. A. Tissue explants from NP or tonsil were cultured in vitro for 4 days, and total antibody production was measured from the culture supernatants. NP produced significantly higher levels of all antibody isotypes compared to tonsil. B. The frequency of antibody-secreting cells was determined by ELISpot assays. Representative triplicate ELISpot wells from NP are shown; arrows in the IgE wells indicate representative positive spots. NP contained significantly elevated frequencies of IgG-, IgA-, and IgE-secreting cells compared to tonsil. C. Expression of AID and germline transcripts was measured using qRT-PCR. AID expression was highest in tonsil, but detectible in NP. Expression of germline transcripts for IgE, IgG1, IgG4, IgA1 and IgA2 was detectible in NP. Data represent mean ± SEM; *p<0.05, **p<0.01; ***p<0.001 by Mann-Whiney U test.