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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: J Thromb Haemost. 2017 Feb 25;15(4):802–813. doi: 10.1111/jth.13620

Figure 1. Human platelets and the megakaryocyte cell line, Meg01, express the SPM receptors, GPR32 and ALX.

Figure 1

Washed human platelets were generated from leukoreduced whole blood from healthy human donors. Platelets were blocked with human Fc Receptor blocking reagent for 30 minutes, then stained with rabbit anti-human GPR32 followed by rabbit FITC secondary antibody (A–B) or rabbit anti-human fpr1-FITC (D–E) antibodies for one hour. Grey shaded histogram represents the isotype control. Mean +/− SEM n=3. The mean fluorescence intensity (MFI) for three independent human donors is shown for GPR32 (B) and ALX (E). The MFI for GPR32 was determined by subtracting the MFI from secondary only control. (C, F) Lysates were generated from washed human platelets, the megakaryocyte Meg01 cell line, A549 cells (GPR32 positive control), or HEK cells (ALX positive control). Proteins were separated on two separate 10% SDS-PAGE gels and probed for GPR32 or ALX. One representative donor is shown.

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