Figure 2. SPMs mitigate platelet aggregation.
PRP was generated from healthy human donors and treated with 100 nM SPMs (Lipoxin A4, LXA4; Resolvin D1, RvD1; Resolvin D2, RvD2; 17HDHA; and Maresin 1, Mar1) for 15 minutes prior to initiation of aggregation by 5 μM ADP (A–D). Aggregation was measured for five minutes and the percent change in maximum amplitude was calculated for each SPM relative to vehicle control (0.1% EtOH) (E). Statistical significance was determined by One-way ANOVA with Dunnett’s multiple comparison post-test. *p<0.05, ***p<0.001