Figure 3. SPMs mitigate platelet spreading.

Washed platelets were generated from healthy human donors and treated with vehicle or 100 nM SPMs for 15 minutes then were allowed to spread on fibrinogen-coated coverslips for 45 minutes. Coverslips were fixed and visualized by differential interference contrast (DIC) optics using an Olympus BX51 microscope at 100X and SPOT computer software. Scale bars = 10 microns. One representative donor of 5 is shown (A–F). The percentage of fully spread platelets per field of view was determined by manual counting four fields of view for 5 individual donors and the percent change in spreading was calculated for each SPM relative to vehicle control (0.1% EtOH). (E). Mean +/− SEM. n=5 Statistical significance determined by One-Way ANOVA with Dunnett’s multiple comparison post-test. *p<0.05, ***p<0.001