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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: J Thromb Haemost. 2017 Feb 25;15(4):802–813. doi: 10.1111/jth.13620

Figure 4. SPMs differentially affect mediator release from unactivated and activated human platelets.

Figure 4

Freshly isolated washed platelets from healthy human donors were treated with vehicle (0.1% DMSO), SPMs for 30 minutes then were either left unactivated, or activated with 5 μM ADP or 0.1U/mL thrombin for 15 minutes. Supernatants were collected and analyzed for PF4 or sCD40L release by ELISA (A–B) or platelets were assessed for CD62P positivity by flow cytometry (C). Mean +/− SEM. n=4 Statistical significance was determined by Two-Way RM ANOVA with Dunnett’s multiple comparison post-test.

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