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. 2017 Apr 4;7:111. doi: 10.3389/fcimb.2017.00111

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Copyright © 2017 Zhou, Fang, Wang, Cai, Chen and Xiao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Role of SGs in the PRRSV-induced inflammatory responses. (A) MARC-145 cells were simultaneously transfected with siTIA1, siG3BP1, and siTIAR for 24 h, and cells were then mock-infected or infected with PRRSV. Cytokine mRNA levels (IL-6, IL-8, and TNF-α) were evaluated using real-time RT-qPCR assays at 36 hpi. ^***P < 0.01, comparison between cells transfected with NC siRNA and specific siRNA followed by PRRSV infection. (B) MARC-145 cells are treated as described for (A). At 36 hpi, the levels of p65 phosphorylation were evaluated by Western blotting. The ratio of phosphorylated/total p65 was analyzed using ImageJ Software.