Figure 3.

The eukaryotic 26S does not stall when it encounters the folded domain of GFP. (A) PAN's ATPase rate was slowed by competing with increasing ratios of ATPγS:ATP (2 mM total nucleotide), and GFPssrA (0.2 μM) unfolding rate was assessed as in Figure 1A. Data fit a line with an R² = 0.989. (B) 25 nM of purified rabbit 26S was incubated with 100 nM Ub⁴(lin)-GFP³⁵ and was analyzed as in (A). Data fit a line with an R²-value of 0.982. “Stalling” is defined in Figure 2. Error bars are standard deviations from three independent experiments (n = 3).