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. 2017 Apr 4;8:388. doi: 10.3389/fimmu.2017.00388

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Copyright © 2017 Zhang, Wang, Zhang, Chen, Nie, Li and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Constitutive expression of antimicrobial peptide (AMP) genes in trout IgM⁺, IgT⁺, and DN lymphocytes as well as myeloid leukocytes. (A) Flow cytometry of trout peripheral blood leukocytes (PBLs) and head kidney leukocytes (HKLs) double stained with mouse anti-trout IgM and anti-trout IgT monoclonal antibodies. Lymphocytes (Lym) and myeloid leukocytes (Mye) were gated, and the IgM⁺, IgT⁺, and IgM⁻IgT⁻ (DN) lymphocytes as well as myeloid leukocytes were FACS sorted and subjected to total RNA isolation and cDNA synthesis. (B) Expression patterns of AMP genes in trout IgM⁺, IgT⁺, and DN lymphocytes as well as myeloid leukocytes analyzed by normal RT-PCR. (C) Expression levels of AMP genes in trout IgM⁺, IgT⁺, and DN lymphocytes as well as myeloid leukocytes analyzed by quantitative real-time PCR and normalized against the expression of elongation factor 1a (EF-1a). Data are representative of three independent experiments [mean ± SD in panel (C)].