Figure 5.

In vivo-induced expression of antimicrobial peptide (AMP) genes in trout B cells by Aeromonas salmonicida. Healthy trout were injected intraperitoneally with PBS (Ctrl) or A. salmonicida (A.sal). The IgM⁺ and IgT⁺ B cells were MACS sorted from peripheral blood leukocytes (PBLs) and head kidney leukocytes (HKLs) of trout at 30 h postinfection and then subjected to total RNA isolation and cDNA synthesis. The relative expression levels of AMP genes in the IgM⁺ and IgT⁺ B cells from healthy and infected trout were determined by quantitative real-time PCR and normalized against elongation factor 1a (*p < 0.05). Data are representative of three independent experiments (mean ± SD).