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. 2016 May 12;16(2):195–218. doi: 10.1007/s11101-016-9470-4

Table 6.

Maximal contents (mg/100 g DW) of dibenzocyclooctadiene lignans obtained by different research teams under biotechnological studies on in vitro cultures of S. chinensis

Lignans Maximal content Type of in vitro culture Medium and PGRs References
Angeoyl-/Tigloylgomisin H 26.66 Shoot-differentiating callus agar culture MS
BA—3 mg/l
NAA—1 mg/l
Szopa et al. (2016a)
Angeoyl-/Tigloylgomisin Q 51.24 Shoot-differentiating callus culture MS
BA—3 mg/l
NAA—1 mg/l
Szopa et al. (2016a)
Benzoylgomisin P 18.74 Stationary liquid shoot culture MS
BA—3 mg/l
NAA—1 mg/l
Szopa et al. (2016a)
Deoxyschisandrin 308.51 Shoot-differentiating callus culture MS
BAP—3 mg/l
NAA—1 mg/l
Szopa and Ekiert (2013)
Gomisin A 86.41 Shoot-differentiating callus culture MS
BAP—3 mg/l
NAA—1 mg/l
Szopa and Ekiert (2011)
Gomisin F 40.00 Callus solid culture ½ MS
KIN—0.05 mg/l
2,4-D—0.2 mg/l
Kohda et al. (2012)
Gomisin G 1.91 Shoot agitated culture MS
BA—3 mg/l
NAA—1 mg/l
Szopa et al. (2016a)
Gomisin N 54.70 Embriogenic callus
(cell line: LAT-52)
Westvaco
BAP—0.9 mg/l
2,4-D—2.21 mg/l
Brezinová et al. (2010)
Schisandrin 70.54 Shoot-differentiating callus culture MS
BAP—3 mg/l
NAA—1 mg/l
Szopa and Ekiert (2011)
Schisandrin B 20.70 Shoot agitated culture MS
BA—3 mg/l
NAA—1 mg/l
Szopa et al. (2016a)
Schisandrin C 20.90 Suspension culture
(cell line: LA-1)
Westvaco
BAP—0.9 mg/l
2,4-D—2.21 mg/l
Brezinová et al. (2010)
γ-Schisandrin 55.00 Suspension culture
(cell line: LA-1)
Westvaco
BAP—0.9 mg/l
2,4-D—2.21 mg/l
Brezinová et al. (2010)
Schisanthenol 1.28 Shoot agitated culture MS
BA—3 mg/l
NAA—1 mg/l
Szopa et al. (2016a)
Schisantherin A 2.97 Shoot agitated culture MS
BA—3 mg/l
NAA—1 mg/l
Szopa et al. (2016a)
Schisantherin B 5.70 Shoot agitated culture MS
BA—3 mg/l
NAA—1 mg/l
Szopa et al. (2016a)
Schisantherin D 5.65 Stationary liquid shoot culture MS
BA—3 mg/l
NAA—1 mg/l
Szopa et al. (2016a)