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Validation of selected miRNAs with quantitative real-time PCR (qRT-PCR). Fold difference of expression for miR-22 (P < 0.05), miR-122 (P < 0.05), and miR-320a (P < 0.05) in female versus male-conditioned culture medium. Error bars represent SE for the mean fold change of the expression range. Asterisk denotes significant difference between males and females.
