FIGURE 2.

Effects of gabapentin on cytokines production by lipopolysaccharide (LPS) in corneal cells. SIRC were pre-incubated in serum-free medium (SFM) for 1 h with gabapentin (10 μg/ml), and the inflammatory stimulus was induced with LPS (1 μg/ml) for 24 h. Lysates were prepared for Western blot analysis as described in section “Materials and Methods.” BOX: Effect of LPS on SIRC viability after 24 h incubation; for the subsequent experiments was chosen the 1 μg/ml not toxic dose. (A) Representative immunoblot images of TNF-α, IL-1β, and β-actin following LPS treatment with or without gabapentin. (B,C) Densitometric analysis of TNF-α and IL-1β protein levels after adjusting for β-actin band intensity. Values represent mean ± SD, ^∗p < 0.05 vs. LPS, ^xp < 0.01 vs. CTRL. One-way ANOVA, followed by Tukey’s test.