Table 2. Normalized least-squares error for the best fit of model types to the binding curves for AT1R-H8 binding to lipid bilayers at 10 μM peptide concentration.
Lipid composition | 2-state | 2-state + expansion | 2-state + expansion + lag | 2-state + expansion + threshold | 3-state | 3-state + expansion | 3-state + expansion + lag | 3-state + expansion + threshold |
---|---|---|---|---|---|---|---|---|
DMPC | 0.389 | 0.356 | 0.356 | 0.249 | 0.177 | 0.177 | ||
DMPC/DMPS (80:20) | 0.105 | 0.104 | 0.103 | 0.085 | 0.056 | 0.056 | ||
DMPC/DMPS/PI(4)P (76:20:4) | 2.168 | 2.168 | 1.545 | 0.919 | 1.554 | 0.796 | 0.319 | 0.303 |
DMPC/DMPS/PI(4,5)P2 (76:20:4) | 0.837 | 0.422 | 0.367 | 0.422 | 0.756 | 0.375 | 0.153 | 0.169 |
“Expansion” refers to the use of bilayer expansion in the model, “Lag” refers to the use of the birefringence-lag model; “Threshold” refers to the use of the mass-threshold model.