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. 2015 Jul 23;5:12465. doi: 10.1038/srep12465

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Mice were implanted with 4T1 cells (5 × 10⁴ cells/mouse) by intravenous injection (A) or orthotopic injection into the mammary fat pads (B). Metastatic colonization of the lungs was measured as described in the Materials and Methods section (n = 10). A significant correlation between poor prognosis and the expression of a negative (GSK3β) or positive (TCF4) regulator of Wnt/β-catenin signaling was observed in human breast cancer datasets obtained through the Oncomine dataset repository (www.oncomine.org) (C). A significant correlation between the risk of recurrence and the expression of a negative (GSK3β) or positive (TCF4) regulator of Wnt/β-catenin signaling was observed in human breast cancer datasets obtained through the Oncomine dataset repository (www.oncomine.org) (C). The results are presented as the mean ± SD, as determined from more than three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.