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. 2015 Jul 23;5:12433. doi: 10.1038/srep12433

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Haploid strains GZY792 (a,b) and GZY803 (c,d) were cultured at 30 °C overnight and re-inoculated to allow the development of biofilm at 37 °C. Samples were taken from both biofilm (a,c) and dispersed cells (b,d) at the time points as indicated for ploidy examination by flow cytometry analysis. SC5314 was used as the standard for diploidy.