Figure 2. GINIP-expressing neurons ablation occurs in a cell specific manner.

(A) Quantification of the total number of L4 DRGs neurons as well as the total number of neurons expressing the main markers of DRGs (n = 3 for each genotype). (***p < 0.001; *p < 0.05). (B) In-situ hybridization on DRG sections using antisense probes for genes that are known to be expressed in GINIP⁺ neurons (red). Each in situ hybridization is followed by immunostaining using rat anti-GINIP (green) to confirm the ablation of GINIP⁺ neurons in GINIP-DTR mice. Scale bar: 100 μm. (C) In-situ hybridization on DRG sections using antisense probes for genes that are known to be excluded from GINIP⁺ neurons (red). Each in situ hybridization is followed by immunostaining using rat anti-GINIP (green) to confirm the ablation of GINIP⁺ neurons in GINIP-DTR mice. Scale bar: 100 μm.