Figure 3.

Scutellarin promotes NSC proliferation

(A) Cultured NSCs were stained with anti-nestin antibody and then additionally stained with antibody against Ki67 to detect active growth or antibody against BrdU to detect DNA synthesis

(B) Quantitative analysis of NSC proliferation (n=5 per group) *P<0.05, **P<0.01 vs vehicle

(C) RT-qPCR analysis of nestin mRNA levels (n=3 per group) *P<0.05 vs vehicle

(D) Western blotting of cell lysates with antibody against nestin.

(E) Quantitative analysis of nestin protein levels in lysates (n=3 per group). *P<0.05 vs vehicle