Figure 5.

Scutellarin promotes neuronal differentiation of NSCs

(A) NSCs were treated with SCU in differentiation medium for 3 days, and then stained with Tuj-1 and GFAP

(B) SCU increased the percentage of cells positive for Tuj-1 and decreased the percentage of cells positive for GFAP (n=3 per group). *P<0.05, **P<0.01 vs vehicle

(C) Lysates from differentiated cells were analyzed by Western blotting with antibody against Tuj-1

(D) Quantitative analysis of blots like those in panel C (n=3 per group). *P<0.05 vs vehicle

(E) RT-PCR analysis of levels of mRNAs encoding Ngn1 and Hes1 (n = 3 per group). *P< 0.05, **P<0.01, ^ P>0.05 vs vehicle