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. 2017 Mar 21;18(12):2836–2844. doi: 10.1016/j.celrep.2017.02.081

Figure 4.

Figure 4

Degradation of B55α by PHD2 Blocks Neoplastic Growth in Response to Glucose Deprivation

(A and B) Colony-forming assay from SKBR3 cells stably silenced for B55α (shB55α) or control (shCTRL). Representative images from one of three independent experiments are shown (B).

(C) In vivo growth curve of xenograft tumors derived from subcutaneous injection of CD1 mice of MDA-MB231 cells stably silenced for control (shCTRL) or PHD2 (shPHD2).

(D) Graph shows tumor weight of MDA-MB231 cells 38 days after cancer cell injection.

(E) Graph shows quantification of B55α protein levels upon WB analysis of tumor samples derived from the experiment in (C).

(F) Morphometric quantification and representative images of TUNEL+ apoptotic cells in MDA-MB231 tumor sections.

Representative images and morphometric quantification of MDA-MB231 tumor sections stained with TUNEL assay, showing cell death. ∗p < 0.05 versus all other conditions in (A) and (C–F). All graphs show mean ± SEM.