Figure 1.

CD11c-Expressing Cells in the Heart Can Be Subdivided into cDC1s, cDC2s, and moDCs

(A) Flow cytometry gating strategy for DC subsets in steady-state heart of WT mice.

(B) Pie chart representing the distribution of DC subsets in naive murine WT heart.

(C) DC subset percentages of total CD11c⁺ cells in naive heart of WT mice.

(D) Expression of MHCII and CD64 in CD45⁺Lineage⁻CD11c⁺ cells from naive heart in Flt3l^+/+ and Flt3l^−/− mice.

(E) Total cDC, cDC1, cDC2, and moDC percentages of total living cells in naive heart of Flt3l^+/+ and Fltl3^−/− mice.

(F) Representative histograms of CD26, Flt3, CD11b, CD103, CD24, CADM1, MerTK, Mar-1, CCR2, and F4/80 expression in steady-state WT heart cDC1s, cDC2s, and moDCs (n = 3).

(G) MFI of marker expression on steady-state WT heart DC subsets shown in (F).

(H and I) Heat map of relative expression of (H) hallmark cDC1 genes and (I) hallmark cDC2 genes in cDC1s, cDC2s, moDCs, and MFs sorted from naive WT hearts acquired by RNA-seq. All data in Figure 1 represent at least two independent experiments, and all bar graphs show data as mean ± SEM (^∗p ≤ 0.05).