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. 2017 Mar 21;18(12):3005–3017. doi: 10.1016/j.celrep.2017.02.079

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© 2017 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

IRF8-Dependent cDC1s Generate Myosin-Specific Tregs in Heart-Draining Lymph Node

(A) Depicted LNs and spleen were isolated from TCR-M acceptor Irf4^fl/fl and Irf4^fl/fl.Cd11cCre mice 3 days after naive TCR-M transfer. CFSE dilution and CD44 expression of donor TCR-M cells was analyzed by flow cytometry.

(B) Percentage of proliferation and CD44 expression of donor TCR-M cells in LNs and spleen from the experiment described in (A) (n = 4).

(C) Percentage of T-bet, RoRγt, and Foxp3/CD25 expression on undivided and divided donor TCR-M cells isolated from mLN in Irf4^fl/fl and Irf4^fl/fl.Cd11cCre steady-state mice (n = 4).

(D) 3 days after naive TCR-M injection, depicted LNs and spleen were isolated from TCR-M acceptor Irf8^fl/fl and Irf8^fl/fl.Cd11cCre mice. CFSE dilution and CD44 expression of donor TCR-M cells was analyzed by flow cytometry.

(E) Percentage of proliferation and CD44 expression of donor TCR-M cells in LNs and spleen from the experiment described in (D) (n = 5).

(F) Percentage of T-bet, RoRγt, and Foxp3/CD25 expression on undivided and divided donor TCR-M cells isolated from mLN in Irf8^fl/fl and Irf8^fl/fl.Cd11cCre steady-state mice (n = 5). All data in Figure 3 represent at least three independent experiments, and bar graphs show data as mean ± SEM (^∗p ≤ 0.05).