Figure 4.

DCs Infiltrate the Heart and Migrate to Mediastinal Nodes Following MI

(A) Representative gating strategy for DC subsets in the heart of sham-operated and infarcted WT mice on day 7 post-surgery.

(B) Absolute cell numbers of DC subsets in the heart of sham-operated and infarcted mice on day 2, 5, 7, and 10 post-surgery (n = 3–6).

(C) Pie charts depicting the distribution of DC subsets in the sham-operated heart (pooled data from day 2-5-7-10 post-surgery) and infarcted heart on day 2, 5, 7, and 10 post-MI. Pie chart sizes are proportional to total cardiac DC percentages.

(D) Representative gating strategy for MHCII^int and MHCII^hi DCs in mediastinal LN of sham-operated and infarcted WT mice on day 7 post-surgery.

(E) MHCII^hi DC percentages of total living cells in mediastinal and mesenteric LN in sham-operated and infarcted mice at day 7 post-surgery (n = 6).

(F) MHCII^hi DC subset percentages of total living cells in mediastinal LN of sham-operated and infarcted mice on day 2, 5, 7, and 10 post-surgery (n = 3–6).

(G) Pie chart illustrating distribution of cDC1s, cDC2s, and moDCs among total MHCII^hi DC population in mLN in sham-operated and infarcted mice at day 2, 5, 7, and 10 post-surgery. Pie chart sizes are proportional to total MHCII^hi DCs in mLN.

(H) Representative histogram of CCR7 expression of MHCII^hi cDC1s, MHCII^hi cDC2s, MHCII^hi moDCs, and MHCII^int cDCs from mLN on day 7. All bar graphs in Figure 4 show data as mean ± SEM (^∗p ≤ 0.05; ^∗∗p ≤ 0.01), and all data are representative of two independent experiments.