Figure 6.

cDC2s Are the Main Presenters of αMyHC to Effector Autoreactive CD4⁺ T Cells Ex Vivo

(A) CFSE dilution and CD25 expression of TCR-M cells in co-culture with sorted heart DC subsets from sham-operated versus MI d7 mice. Data are representative of three independent experiments (n = 12).

(B) Quantification of percentages of proliferated and CD25-expressing TCR-M cells from co-cultures plotted in (A). Individual dots represent the value of one independent experiment (mean ± SEM; ^∗p ≤ 0.05).

(C) Percentage of T-bet, RoRγt, and Foxp3/CD25 expression on TCR-M cells co-cultured with sorted heart DC subsets from MI day 7 hearts (mean ± SEM).

(D) In supernatants of co-cultures plotted in (A), cytokines produced by TCR-M cells were detected by ELISA. Mean is calculated from values of technical replicates from one experiment (nd, not detectable) (mean ± SD).

(E) CFSE dilution and CD25 expression of TCR-M cells co-cultured with sorted migratory cDC1s and cDC2s from mLNs of sham-operated and MI mice and from mesenteric LNs of MI mice.

(F) After 3 days of co-culture (plotted in E), cytokines were measured in supernatants by ELISA. Mean is calculated from values of technical replicates from one experiment (nd, not detectable) (mean ± SD).

(G) Heat map of relative expression of cDC2 genes exclusively upregulated in cDC2s from the infarcted heart compared to steady state.

(H) Bar graphs representing absolute expression of interesting unique cDC2 DEGs (mean ± SEM; ^∗p ≤ 0.05).

See also Table S8.