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. 2017 Mar 21;18(12):3005–3017. doi: 10.1016/j.celrep.2017.02.079

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© 2017 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Myocardial Infarction Activates Autoreactive CD4⁺ T Cells In Vivo

(A) TCR-M cells were injected into sham or MI mice on day 2 or day 7 post-surgery. CFSE dilution and CD44 expression of donor TCR-M cells is shown.

(B) Proliferation of donor TCR-M cells in lymphoid organs from the experiment described in (A).

(C) Percentage of Foxp3/CD25 expression on undivided and divided donor TCR-M cells isolated from mLN in sham and MI mice at day 2 post-surgery.

(D) CFSE dilution and CD44 expression of naive TCR-M cells in ex vivo co-culture with bulk mediastinal LN, spleen, and mesenteric LN cells isolated from sham and MI mice on day 2 and day 7 post-surgery.

(E) Heart cDC2 and migratory cDC2 percentages in mLN of sham and MI Irf4^fl/fl and Irf4^fl/fl.Cd11cCre mice at day 7 post-surgery.

(F) Irf4^fl/fl, Irf4^fl/fl.Cd11cCre, Irf8^fl/fl and Irf8^fl/fl.Cd11cCre mice were injected with TCR-M cells at day 2 post MI. CFSE dilution and CD44 expression of donor TCR-M cells is shown.

(G) Proliferation and CD44 expression of donor TCR-M cells in LNs and spleen from Irf4^fl/fl and Irf4^fl/fl.Cd11cCre mice from the experiment described in (F).

(H) Heart cDC percentages of sham and MI Irf8^fl/fl and Irf8^fl/fl.Cd11cCre mice at day 7 post-surgery.

(I) Proliferation and CD44 expression of donor TCR-M cells in LNs and spleen from Irf8^fl/fl and Irf8^fl/fl.Cd11cCre mice from the experiment described in (F). All bar graphs in Figure 7 show data as mean ± SEM (^∗p ≤ 0.05; ^∗∗p ≤ 0.01; ^∗∗∗p ≤ 0.001), and all data are representative of two independent experiments.