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Example sample mounting techniques for live tissue imaging for both upright and inverted imaging geometries. (a) High-resolution images may be acquired in windowed fly pupae mounted against a coverglass and may be imaged from above or below by inverting the slide. (b) An array of samples mounted on a gel pad braced against the edges of a 60 mm tissue culture dish. (c) Inverted culture system for imaging samples through a coverglass or gas-permeable Lumox® dish.
