Figure 4. 2-AG augmentation promotes resilience to acute stress-induced anxiety-like behaviour.

(a) Home cage training (blue lines) and novel cage (NC) latencies and (b) consumption before (NC-V) and after (NC-FS-V) foot-shock stress with vehicle (V), JZL-184, and JZL-184+Rimonabant (RIM) treatment. (c) Resilient subgroup latencies separated from a. (d) Resilient individuals' baseline and post-stress latencies and consumption. (e) Susceptible subgroup latencies separated from a. (f) Susceptible individuals' baseline and post-stress latencies and consumption. (g) Direct comparison of changes in latency from baseline between the 2nd stress test with JZL-184 (NC-FS2-JZL) and the first stress test with vehicle (NC-FS1-V). (h) Correlation between stress-test latency and change in latency between JZL-184 and vehicle treatment for susceptible individuals. (i) Stress-susceptibility ratios for the same cohort of mice across three weeks after vehicle, JZL-184, or JZL-184+Rimonabant treatment. (j) Home cage testing latency (left) and consumption as % of previous day's home cage/no stress consumption (right) 24 h after stress exposure with resilient (black circles) and susceptible (red circles) individuals treated with vehicle (VEH) or JZL-184 (blue) one week after susceptibility categorization. (k) Whole population correlations between post-stress novel cage test feeding latency and consumption with vehicle and JZL-184 treatment. Blue arrows indicate foot-shock stress exposure. F and P values for one-way (a–c,e) or two-way (g) ANOVA shown above individual panels. P values for pairwise comparisons derived from Holm-Sidak multiple comparisons test after ANOVA, unpaired two-tailed t-test (j), or paired two-tailed t-test (d,f) shown in panels. R² and P value for linear regression shown in h,k. P values from chi-squared tests reported in i. Data are presented as mean±s.e.m.