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. 2017 Apr 4;8:558. doi: 10.3389/fmicb.2017.00558

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Copyright © 2017 Alfán-Guzmán, Ertan, Manefield and Lee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Native-PAGE gel with molecular weight marker (left lane), silver stain samples (center lane) and lettered segments indicating gel slices excised and subjected to dechlorinating activity assays (right lane) (Sections on the right side of the gel indicate how a full lane was excise for LC/MS/MS analysis). Dechlorinating activity assay using eluted proteins from unstained excised gel slices with either 1,2,4,5-TeCB (B) or 1,2,4-TCB (C), in both cases the shown concentrations correspond to the dechlorination end products. The positive control was performed using the crude protein extract.