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. 2017 Mar 30;8:14770. doi: 10.1038/ncomms14770

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(a) Ventral scanning electron micrograph of the DPW epithelium of an E9.5 embryo after removing the heart. Apical surface view of the DPW at E9.5 showing F-actin (b), apical membrane skeleton after segmentation (c) and a heat map of apical surface area (d). (e) Cells in the pDPW have a larger apical surface area and are more elongated than in the aDPW (n=3 embryos, pDPW: 1,522 cells, aDPW: 1,688 cells). (f) Cells are elongated on an axis oriented towards the arterial pole (red bar) (n=3 embryos, right: 1,462 cells, left: 1,671 cells; P-value based on a Rayleigh circular test). OFT: outflow tract, VP: venous pole. ***P<0.001 (e: Unpaired bilateral t-test; f: Chi-squared test). Error bars represent s.e.m. Scale bars, 30 μm.