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. 2017 Mar 10;9(1):43–52. doi: 10.1038/ijos.2016.57

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Effects of PCM molecules on NO and MMP-13 production. Condylar chondrocytes (a, c) and disc chondrocytes (b, d) pre-treated with collagen VI, collagen IV or laminin were subjected to IL-1β-induced inflammation for 24 h. NO concentration in culture supernatant was measured using the Griess reaction (a, b). MMP-13 level was measured using ELISA and normalized to the total protein concentration (c,d). Mean±s.e.m.; n=3. ^#P<0.05, ^###P<0.001, compared with untreated control. *P<0.05, **P<0.01, ***P<0.001, compared with IL-1β-treated group. ELISA, enzyme-linked immunosorbent assay; IL-1β, interleukin-1β MMP, matrix metalloproteinase; NO, nitric oxide; PCM, pericellular matrix; s.e.m.: standard error of the mean.