Figure 4. Characterization of BurrH-based nucleases in vivo (293H).

(a) Alignment of the WT genomic sequence and the most predominant events (deletions are indicated by dashes) induced by the BurrH-based nuclease (20 modules, bn21604/21608) at the CAPNS1 locus. Binding sites of the two half BurrH-based nuclease are underlined. (b) Size distribution of the deletion (blue bars) and insertion (red bars) events induced at the CAPNS1 locus by the BurrH-based nuclease composed of 20 modules. (c) Targeted gene insertion frequency determined at the CAPNS1 locus in the presence (+) or absence (-) of the nuclease. Error bars denote s.d.; n = 2.