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. 2017 Apr 4;7:45827. doi: 10.1038/srep45827

Figure 1. High glucose induces PON1 glycation and ER stress.

Figure 1

(A–C) Recombinant PON1 protein (10 μg) was incubated with D-glucose (5, 15, 30 mM) for 7 days in reaction buffer. The level of glycated PON1 was determined by running and staining SDS-PAGE with coomassie brilliant blue in (A) and was calculated in (B). PON1 activity was measured in (C). N = 3 per group. *P < 0.05 vs. Control (5 mM D-glucose). (D) Cultured HUVECs were incubated with native or glycated PON1 (10 μg/ml) as indicated times (0.5–12 hours). (E) HUVECs were incubated with glycated PON1 as indicated concentrations (2.5, 5, 10, 20 μg/ml) for 6 hours. The levels of p-PERK, p-eIF2α, CHOP, ATF6 and BIP were assayed by western blot in total cell lysates from (D,E). The blot was a representative picture from 3 independent experiments and cropped from the full blot shown in Supplementary Figures S1, S2 and S3.