Table 2. Parameters for voltage-dependence of activation and inactivation of Nav1.2 (α + β1) expressed in Xenopus laevis oocytes.
| BmK AS | Administration2 | Activation | Steady-state inactivation | ||||
|---|---|---|---|---|---|---|---|
| Concentration1 | V1/2 (mV) | km (mV) | N3 | V1/2 (mV) | km (mV) | N | |
| 1 nM | Control (Pre AS) | −28.66 ± 0.52 | 2.91 ± 0.34 | 5 | −53.10 ± 0.42 | 5.82 ± 0.21 | 6 |
| Pre AS | −26.61 ± 0.42* | 2.89 ± 0.65 | 6 | −53.46 ± 0.41 | 5.80 ± 0.24 | 6 | |
| Pro Iso | −26.11 ± 0.37** | 2.84 ± 0.32 | 6 | −53.12 ± 0.55 | 6.14 ± 0.28 | 6 | |
| Control (Pro AS) | −26.65 ± 0.79 | 4.645 ± 0.29 | 6 | −53.25 ± 0.35 | 5.51 ± 0.18 | 6 | |
| Pre Iso | −25.40 ± 2.16 | 4.14 ± 0.67 | 6 | −53.66 ± 0.57 | 5.85 ± 0.32 | 6 | |
| Pro AS | −24.56 ± 0.82 | 4.53 ± 0.25 | 5 | −54.72 ± 0.51* | 6.61 ± 0.34* | 6 | |
| 10 nM | Control (Pre AS) | −28.94 ± 1.05 | 3.07 ± 0.55 | 5 | −54.33 ± 0.43 | 5.22 ± 0.51 | 7 |
| Pre AS | −22.91 ± 0.82** | 3.70 ± 0.90 | 6 | −57.36 ± 0.31*** | 5.90 ± 0.15 | 5 | |
| Pro Iso | −22.38 ± 0.78** | 3.41 ± 2.00 | 4 | −53.75 ± 0.24 | 5.79 ± 0.12 | 4 | |
| Control (Pro AS) | −29.15 ± 1.35 | 2.61 ± 0.49 | 8 | −52.53 ± 0.43 | 5.14 ± 0.23 | 8 | |
| Pre Iso | −29.29 ± 0.87 | 3.34 ± 0.32 | 7 | −52.10 ± 0.46 | 6.05 ± 0.28 | 6 | |
| Pro AS | −24.81 ± 1.31* | 4.28 ± 0.45* | 8 | −53.55 ± 0.36 | 6.13 ± 0.34* | 8 | |
| 100 nM | Control (Pre AS) | −29.68 ± 0.58 | 4.45 ± 0.61 | 6 | −52.87 ± 0.11 | 6.12 ± 0.13 | |
| Pre AS | −23.25 ± 0.12*** | 3.78 ± 0.10 | 6 | −56.91 ± 0.09*** | 7.01 ± 0.06*** | 6 | |
| Pro Iso | −19.49 ± 0.32*** | 5.03 ± 0.28 | 6 | −54.84 ± 0.12*** | 6.04 ± 0.10 | 6 | |
| Control (Pro AS) | −29.11 ± 3.50 | 2.40 ± 1.21 | 5 | −54.20 ± 0.36 | 6.23 ± 2.09 | 7 | |
| Pre Iso | −28.57 ± 1.80 | 2.60 ± 0.59 | 4 | −54.62 ± 0.45 | 5.79 ± 0.29 | 7 | |
| Pro AS | −26.17 ± 0.41 | 3.35 ± 0.14 | 4 | −54.55 ± 0.42 | 6.24 ± 0.36 | 5 | |
1Kinetic parameters of Nav1.2 (α + β1) after applying with BmK AS of different concentrations (1, 10 and 100 nM) were listed as Mean ± SEM separately.
2All the concentrations of BmK AS were applied before (Pre AS) and after (Pro AS) PKA activation through perfusion with 100 μM Iso.
3N indicates the number of samples tested.
*, ** and *** indicates significant difference between control and BmK AS or Iso treated oocytes (*, P < 0.05; **, P < 0.01; ***, P < 0.001; t-test).