Figure 3. Rarefaction curves for species-level TM7 OTUs (0.02 similarity) recovered using new taxon-specific primers and 454 bar coded tag pyrosequencing.

Each group of samples was sequenced to various depths with human oral samples sequenced to asymptote. The higher species richness of environmental samples, particularly soil and seal faecal samples is highlighted with the Australian soil requiring much more sequencing then the other environments.