Table 3. Summary of the BLAST searches using mitochondrial 12S and 16S rRNA gene PCR amplicons from 12 animals, including 2 double-blinded samples (×1 = Rat, ×2 = Frog) and 9 known DNA samples from ZYAGEN. The No.1 BLAST hit corresponds to the first alignment result with the highest identity with the mitochondrial genes. The No.100 BLAST hit corresponds to the last alignment result (out of 100 results) with the lowest identity with the mitochondrial genes. 12Sf and 16Sf represent the forward sequences of the mitochondrial 12S rRNA gene and 16S rRNA gene PCR amplicons, respectively.

Tissues	No. 1 BLAST hit/% Identities	No. 100 BLAST hit/% Identities
Cow	Bos taurus isolate Rom498 mitochondrion, complete genome12Sf, Identity, 100%.Bos taurus isolate PRI18 mitochondrion, complete genome16Sf. Identity, 99%.	Bos taurus isolate PRI18 mitochondrion, complete genome12Sf. Identity, 98%.Bos taurus isolate H1065 mitochondrion, complete genome16Sf. Identity, 99%.
Fly	Muscina stabulans 12S ribosomal RNA gene, partial sequence; mitochondrial12Sf. Identity, 99%.Muscina stabulans 16S ribosomal RNA gene, partial sequence; mitochondrial16Sf. Identity, 99%.	Norellia striolata 12S ribosomal RNA gene, partial sequence; mitochondrial12Sf Identity, 93%.Hemipyrellia ligurriens isolate CSU0907186206A 16S ribosomal RNA gene, partial sequence; mitochondrial16Sf. Identity, 92%.
×2 (Frog)	Rana catesbeiana mitochondrial DNA, complete genome12Sf. Identity, 99%.Rana catesbeiana mitochondrial DNA, complete genome16Sf. Identity, 100%.	Rana chiricahuensis isolate JAC 26724 12S ribosomal RNA gene, partial sequence; mitochondrial12Sf. Identity, 92%.Rana tagoi tagoi mitochondrial gene for 16S rRNA, complete sequence, specimen_voucher: KUHE:3698216Sf. Identity, 92%.
Fish	Pangasianodon hypophthalmus mitochondrion, complete genome12Sf. Identity, 99%.Pangasianodon hypophthalmus isolate MKPDH 01 12S ribosomal RNA gene, partial sequence; tRNA-Val (trnV) gene, complete sequence; and 16S ribosomal RNA gene, partial sequence; mitochondrial16Sf. Identity, 99%.	Pangasius nasutus isolate INKPPSN 01 12S ribosomal RNA gene, partial sequence; tRNA-Val (trnV) gene, complete sequence; and 16S ribosomal RNA gene, partial sequence; mitochondrial12Sf. Identity, 98%.Hemibagrus macropterus voucher SWU10090910 mitochondrion, complete genome16Sf. Identity, 93%.
Rabbit	Oryctolagus cuniculus complete mitochondrial genome12Sf. Identity, 100%.Oryctolagus cuniculus complete mitochondrial genome16Sf. Identity, 99%.	Capra hircus isolate V07-146 mitochondrion, complete genome12Sf. Identity, 88%.Mesoplodon densirostris isolate SWFSC ID z0074263 mitochondrion, partial genome16Sf. Identity, 85%.
×1 (Rat)	Rattus norvegicus mitochondrion, complete genome12Sf. Identity, 99%.Rattus norvegicus strain OXYS mitochondrion, complete genome16Sf Identity, 100%.	Mus musculus isolate M3 mitochondrion, complete genome12Sf. Identity, 92%.Mus musculus mitochondrial DNA, complete genome, clone: P29mtC3H16Sf Identity, 90%.
Eel	Monopterus albus mitochondrial DNA, complete genome12Sf. Identity, 88%. (500bp)Monopterus sp. C-TC-2002 haplotype 1Nanning large subunit ribosomal RNA gene, partial sequence; mitochondrial gene for mitochondrial product16Sf. Identity, 100%.	Enterobacter sp. SWg2 16S ribosomal RNA gene, partial sequence12Sf. Identity, 100%. (200bp)Lutjanus erythropterus mitochondrion, complete genome16Sf. Identity, 83%.
Human	Homo sapiens haplogroup U4a3 mitochondrion, complete genome12Sf. Identity, 100%.Homo sapiens haplogroup I2 mitochondrion, complete genome16Sf. Identity, 100%.	Homo sapiens isolate Kny981_Kankanaey mitochondrion, complete genome12Sf. Identity, 100%.Homo sapiens isolate Kny982_Kankanaey mitochondrion, complete genome16Sf. Identity, 100%.
Mouse	Mus musculus mitochondrial DNA from Lewis lung carcinoma, complete sequence12Sf. Identity, 100%.Mus musculus mitochondrial DNA, complete genome, clone: P29mtC3H16Sf. Identity, 100%.	Mouse mitochondrion, complete genome12Sf. Identity, 100%.Mus musculus domesticus isolate Ker_Jack0912 mitochondrion, complete genome16Sf. Identity, 100%.
Chicken	Gallus gallus isolate Gallus2 12S ribosomal RNA gene, partial sequence; mitochondrial12Sf. Identity, 100%.Gallus gallus murghi 16S ribosomal RNA gene, partial sequence; mitochondrial16Sf. Identity, 99%.	Gallus gallus isolate Gallus2 12S ribosomal RNA gene, partial sequence; mitochondrial12Sf. Identity, 100%.Gallus gallus murghi 16S ribosomal RNA gene, partial sequence; mitochondrial16Sf. Identity, 99%.
Pig	Sus scrofa isolate KNP1501 mitochondrion, complete genome12Sf. Identity, 100%.Sus scrofa isolate KNP1501 mitochondrion, complete genome16Sf. Identity, 100%.	Sus scrofa breed Nuogu mitochondrion, complete genome12Sf. Identity, 98%.Sus scrofa breed Nuogu mitochondrion, complete genome16Sf. Identity, 98%.
Shrimp	Litopenaeus vannamei mitochondrion, complete genome12Sf. Identity, 99%.Litopenaeus vannamei mitochondrion, complete genome16Sf. Identity, 98%.	Litopenaeus vannamei mitochondrion, complete genome12Sf. Identity, 98%.Litopenaeus vannamei mitochondrion, complete genome16Sf. Identity, 96%.