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. 2017 Apr 4;7:45709. doi: 10.1038/srep45709

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This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Shown as black fibres in Hart’s-stained tissue in representative images of multiparous (A) lamina propria with (B) blood vessels removed from analysis and in the (C) muscularis with (D) blood vessels removed from analysis. (E) Graph showing the image analysis quantification of elastic fibre area in tissue images with blood vessels electronically removed. n = 6, primiparous and multiparous n = 8 vaginal tissues/group. Data are mean +/− SEM, *p < 0.05, **p < 0.01, ***p < 0.001.