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Relative gene expression of the Hc-AFP1–4 transcripts in the root tip and associated border-like cells (BLCs) of Heliophila coronopifolia measured using qRT-PCR. Expression was confirmed using the LRE method (Rutledge, 2011) as well as the housekeeping gene Hc-EF1α (De Beer and Vivier, 2011; Pabinger et al., 2014). Data represent the mean and standard deviation for three independent biological repeats performed in triplicate.
