Fig. 7.

Histopathological findings in animals inoculated with MWNT-DENV3E in the presence or absence of adjuvant. 10 weeks old female Balb/c mice were inoculated with the nanoconjugate in the presence or absence of freund complete adjuvant. Seven days after inoculation fragments of dermal and muscular tissues surrounding the injection sites were fixed and prepared for histopathological analyses using hematoxylin and eosin dies. Animals inoculated with PBS or PBS plus adjuvant were used as controls. A, B are representative of animals inoculated with PBS plus adjuvant (PBS CF); C, D are representative of animals inoculated with MWNT-DENV3E in the presence of adjuvant (NT CF); and E, F are representative of animals inoculated with MWNT-DENV3E in the absence of adjuvant (NT SF). Upper and lower panels are figures with different magnifications. Scale bars are included. The table represents an interpretation of the histopathological panels, where − is absence of alterations; + indicates light alterations; ++ indicate moderate alterations; and +++ indicate intense alterations. Figures from animals inoculated with PBS only (PBS SF) are not shown